Although NGS technologies revolutionized the sequencing world, recent efforts have been made to get longer reads. These third-generation methods generate reads of tens of kilobases and are commonly called long-read sequencing method. Long reads have a host of applications48 for which short NGS reads might not be well suited: De novo assembly of large complex genomes, studying complex repetitive regions such as centromeres or telomeres or detection of structural variants. They have recently been used to assemble the first truly complete human genome, including telomeric and centromeric regions4.
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